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Ashwitha, K.
- Characterization and Evaluation of Two Indigenous Bacillus thuringiensis Isolates against Helicoverpa armigera Hubner
Abstract Views :269 |
PDF Views:195
Authors
R. Rangeshwaran
1,
K. Veenakumari
1,
Pritam Karmakar
1,
K. Ashwitha
1,
G. Sivakumar
1,
Satendar Kumar
1
Affiliations
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
1 National Bureau of Agriculturally Important Insects, Post Bag No. 2491, H. A. Farm Post, Bellary Road, Hebbal, Bangalore 560 024, Karnataka, IN
Source
Journal of Biological Control, Vol 25, No 4 (2011), Pagination: 286-293Abstract
Two isolates of Bacillus thuringiensis isolated from dead lepidopteran larvae from a tea garden in Jorhat, Assam and one isolated from soil sample from Rajasthan, obtained in a nationwide screening program showed bipyramidal crystal morphology. These two isolates named as NBAII-BTAS and NBAII-BTG4 were characterized by their high level of toxicity against diamond back moth (Plutella xylostella). The PCR amplification of these two isolates revealed the expected size of the PCR product for cry1Aa, cry1Ab, cry1Ac, cry1E, cry1G, cry1I, and cry2 of 390 bps, 1111bps, 238 bps, 540 bps, 300 bps, 468 bps, and 1170 bps respectively. Purified cry proteins from each of these two cultures were subjected to SDS-PAGE analysis, where, two distinct bands of 130–140 Kda and 65 Kda corresponding to cry1 and cry2 proteins were observed. Toxicity studies was carried out using trypsin activated purified proteins against Helicoverpa armigera, where NBAII-BTG4 derived crystal proteins displayed more toxicity (0.93µg\ml) than NBAII-BTAS.Keywords
Cry Genes, PCR, Specific Cry Primers, Bipyramidal, Toxicity.References
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- Characterization of Abiotic Stress Tolerant Pseudomonas Spp. Occurring in Indian Soils
Abstract Views :376 |
PDF Views:130
Authors
K. Ashwitha
1,
R. Rangeshwaran
1,
N. V. Vajid
2,
G. Sivakumar
1,
S. K. Jalali
1,
K. Rajalaksmi
3,
H. Manjunath
4
Affiliations
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
1 National Bureau of Agriculturally Important Insects, H. A. Farm Post, Hebbal, Bellary Road, Bangalore 560 024, Karnataka, IN
2 Department of Biology, University of Hail, Jeddah, SA
3 Department of Plant Pathology, University of Agricultural Sciences, Dharwad 580 005, Karnataka, IN
4 Department of Plant Pathology, GKVK, UAS Bangalore 560 065, IN
Source
Journal of Biological Control, Vol 27, No 4 (2013), Pagination: 319–328Abstract
Abiotic stress tolerance of 230 Pseudomonas spp. Occurring in Indian soils was evaluated for tolerance to temperature, salinity and moisture stresses. Forty seven Pseudomonas spp. Were characterized as abiotic stress tolerant and were identified as P. aeruginosa (24), P. putida (14), P. plecoglossicida (4), P. mosselli (1), Pseudomonas sp. (1) and P. fluorescens (3). The temperature and salinity tolerance of these bacteria was 45°C and 1 M NaCl respectively. Most isolates (44 out of 47) produced indole acetic acid (IAA) and 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase activity and 37 of them showed phosphatase activity. 2, 4-diacetyl phloroglucinol (DAPG) gene was detected in 10 isolates and pyoluteorin gene was detected in 4 isolates. Under water stress, seed treatment with P. putida (NBAII-RPF9) and P. fluorescens (PFDWD) showed its potential as plant growth promoter. The studies also indicated that stress tolerant Pseudomonas spp. may be used as plant protection agents in abiotically stressed soils.Keywords
Pseudomonas, Abiotic Stress, Tolerance, DAPG, Pyoluteorin.References
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